The Mount Isa Province in northern Australia is one of the world's most strongly endowed regions for base metals and host to major iron-oxide-copper-gold (IOCG) deposits. The Carpentaria Conductivity Anomaly at the eastern margin of the Province is a major electrical conductivity structure of the Australian continent. We have used magnetotelluric and deep seismic reflection data to image the crustal architecture in this complex region to understand the crustal-scale fluid pathways and mineral potential. The resistivity models reveal a number of prominent crustal-scale conductors, suggesting that the Carpentaria Conductivity Anomaly is likely caused by a series of isolated or interconnected bodies. These conductors characterise the position and geometry of the ancient Gidyea Suture Zone, interpreted as a west-dipping subduction zone. The conductivity anomaly may record the activity of fluid hydration involved during a subduction event, with the enhanced conductivity likely being caused by deformation or mineralisation of graphitic or sulfidic rocks during orogenesis. The distribution of known gold and copper deposits shows a close spatial correlation with the suture zone, suggesting that this structure is potentially a fundamental control on IOCG deposits in its vicinity. The interpretation of the seismic image shows a good correlation with the resistivity models. The implication is that crustal-penetrating structures act as potential pathways for fluid movement to form mineral deposits in the upper crust. The significance of mapping such structures using geophysics is highlighted for mineral exploration.